CD40 Ligand Protein, Human, Recombinant

CD40 Ligand Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15 KDa and the accession number is P29965.

规格	价格/CNY		数量
10 μg	￥ 380	5日内发货
50 μg	￥ 1,090	5日内发货
500 μg	￥ 4,860	5日内发货
1 mg	￥ 6,950	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 380

5日内发货

50 μg

￥ 1,090

5日内发货

500 μg

￥ 4,860

5日内发货

1 mg

￥ 6,950

5日内发货

生物活性	1. Loaded Human CD40-His on HIS1K Biosensor, can bind Human CD40L with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L . The ED50 of Human CD40L is 6.05 ng/ml. (Regularly tested)
产品描述	CD40 Ligand Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15 KDa and the accession number is P29965.

种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	P29965
别名	CD40LG, CD40 Ligand, TRAP, TNF-Related Activation Protein, TNFSF5, Tumor Necrosis Factor Ligand Superfamily Member 5, T-Cell Antigen Gp39, CD40-L, CD154, CD40L
氨基酸序列	Met113-Leu261
蛋白构建	Met113-Leu261
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
分子量	15 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

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CD40 Ligand Protein, Human, Recombinant

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