PDGF R beta/CD140b Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 57.25 kDa and the accession number is Q6QNF3.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Human PDGFB, His Tag immobilized on CM5 Chip can bind Canine PDGF R beta, His Tag with an affinity constant of 0.203 μM as determined in SPR assay. |
产品描述 | PDGF R beta/CD140b Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 57.25 kDa and the accession number is Q6QNF3. |
种属 | Canine |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q6QNF3 |
别名 | JTK12, PDGF R β, PDGFR-β, PDGFR, PDGFRB, PDGF R beta, PDGF R β/CD140b, PDGFR1, CD140B, PDGFR-2 |
蛋白构建 | Gly32-Val532 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 57.25 kDa (predicted). Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
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PDGF R beta/CD140b Protein, Canine, Recombinant (His) PDGFR-beta PDGFR 1 JTK12 PDGF R β PDGFR-β PDGFR 2 PDGFR PDGF R b PDGFRB PDGF R beta PDGFR-1 PDGF R β/CD140b PDGFR1 JTK-12 PDGFR2 JTK 12 CD140B PDGFR-b PDGFR-2 recombinant recombinant-proteins proteins protein