Latent TGF-beta 1 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 11 and 35-45 KDa and the accession number is F7HCV5.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 1,020 | 5日内发货 | ||
50 μg | ¥ 3,050 | 5日内发货 | ||
500 μg | ¥ 15,400 | 5日内发货 | ||
1 mg | ¥ 23,100 | 5日内发货 |
产品描述 | Latent TGF-beta 1 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 11 and 35-45 KDa and the accession number is F7HCV5. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | N-6xHis |
蛋白编号 | F7HCV5 |
别名 | 转化生长因子, Transforming growth factor β-1, Latent TGF-β1, TGFB, TGFβ-1, Transforming growth factor beta-1 proprotein, TGFB1 |
氨基酸序列 | Leu30-Ser390 |
蛋白构建 | Leu30-Ser390 (Cys33Ser) |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 11&35-45 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). latent TGF-β1 plays a protective role against bleomycin-induced lung inflammation and fibrosis. The inhibitory effect of latent TGF-β1 on lung inflammation and fibrosis may be associated with the counter-regulatory mechanism between latent and active TGF-β 1, the negative regulatory role of Smad7 in activation of both NF-κB and TGF-β/Smad signaling pathways, and importantly, the GARP-Foxp3 regulatory mechanism in rebalancing the Treg/Th17 response. Some studies have shown that TGFB1 (Cys33Ser) mice develop multiorgan inflammation and tumors consistent with reduced TGF-b1 activity. |
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Latent TGF-beta 1 Protein, Cynomolgus, Recombinant (His) 转化生长因子 Transforming growth factor β-1 Latent TGF-β1 TGFB-1 TGFB 1 TGFB TGFβ-1 Transforming growth factor beta-1 proprotein TGFB1 TGFbeta-1 Transforming growth factor b-1 proprotein Transforming growth factor β-1 proprotein recombinant recombinant-proteins proteins protein