IgG1 hFc Protein, Human, Recombinant, Biotinylated

IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

规格	价格/CNY		货期	数量
20 μg	￥ 2,260	5日内发货
100 μg	￥ 6,130	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,260

5日内发货

100 μg

￥ 6,130

5日内发货

生物活性	1. Loaded Biotinylated Human IgG1 Fc-His-Avi on SA Biosensor, can bind Protein G-Fc with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Biotinylated Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Recombinant Protein G. The ED50 of Recombinant Protein G is 49.41 ng/ml. (Regularly tested)
产品描述	IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

种属	Human
表达系统	HEK293 Cells
标签	C-Avi
蛋白编号	P01857
别名	g gamma-1 chain C region, g γ-1 chain C region, IGHG1
氨基酸序列	Asp104-Lys330(Asp239Glu,Leu241Glu)
蛋白构建	Asp104-Lys330(Asp239Glu,Leu241Glu)
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	33 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

IgG1 hFc Protein, Human, Recombinant, Biotinylated

计算器

技术支持

Keywords