CD28 Protein, Human/Cynomolgus, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 60-90 KDa and the accession number is P10747.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 1,770 | 5日内发货 | ||
100 μg | ¥ 4,340 | 5日内发货 |
生物活性 | Loaded Human B7-1-His on HIS1K Biosensor, can bind Biotinylated Human/Cynomolgus CD28-Fc-Avi with an affinity constant of 9.09 pM as determined in BLI assay. (Regularly tested) |
产品描述 | CD28 Protein, Human/Cynomolgus, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 60-90 KDa and the accession number is P10747. |
种属 | Human,Cynomolyus |
表达系统 | Human Cells |
标签 | C-Fc-Avi |
蛋白编号 | P10747 |
别名 | CD28 molecule, CD28 antigen, CD28, TP44, T-cell-specific surface glycoprotein CD28 |
氨基酸序列 | Asn19-Pro152 |
蛋白构建 | Biotinylated Recombinant Human T-cell-specific surface glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a human IgG1 Fc, Avi tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 60-90 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mTris-HCl, 150mM NaCl, pH 8.0. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
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CD28 Protein, Human/Cynomolgus, Recombinant (hFc & Avi), Biotinylated CD28 molecule TP 44 CD 28 CD-28 CD28 antigen CD28 TP44 T-cell-specific surface glycoprotein CD28 TP-44 recombinant recombinant-proteins proteins protein