Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Cigarette smoking is protective in Parkinson's disease (PD), possibly because of nicotine action on brain nicotinic-acetylcholine receptors. The beta3 nicotinic-acetylcholine receptor subunit (encoded by CHRNB3) is depleted in the striatum of PD patients and associated with nicotine dependence. CHRNB3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.7 kDa and the accession number is Q05901.
生物活性 | Testing in progress |
产品描述 | Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Cigarette smoking is protective in Parkinson's disease (PD), possibly because of nicotine action on brain nicotinic-acetylcholine receptors. The beta3 nicotinic-acetylcholine receptor subunit (encoded by CHRNB3) is depleted in the striatum of PD patients and associated with nicotine dependence. CHRNB3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.7 kDa and the accession number is Q05901. |
种属 | Human |
表达系统 | HEK293 |
标签 | His |
蛋白编号 | Q05901 |
别名 | cholinergic receptor, nicotinic, beta 3 (neuronal), cholinergic receptor, nicotinic, β 3 (neuronal) |
蛋白构建 | A DNA sequence encoding the human CHRNB3 (NP_000740.1) (Met1-Leu232) was expressed with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE. |
分子量 | 25.7 kDa (predicted) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Cigarette smoking is protective in Parkinson's disease (PD), possibly because of nicotine action on brain nicotinic-acetylcholine receptors. The beta3 nicotinic-acetylcholine receptor subunit (encoded by CHRNB3) is depleted in the striatum of PD patients and associated with nicotine dependence. |
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CHRNB3 Protein, Human, Recombinant (His) cholinergic receptor, nicotinic, β 3 cholinergic receptor, nicotinic, b 3 neuronal cholinergic receptor, nicotinic, beta 3 cholinergic receptor, nicotinic, beta 3 (neuronal) cholinergic receptor, nicotinic, β 3 (neuronal) recombinant recombinant-proteins proteins protein