CD45 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 120-160 KDa and the accession number is P06800.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 1,170 | 5日内发货 | ||
50 μg | ¥ 3,450 | 5日内发货 | ||
500 μg | ¥ 17,800 | 5日内发货 | ||
1 mg | ¥ 26,700 | 5日内发货 |
产品描述 | CD45 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 120-160 KDa and the accession number is P06800. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-6xHis |
蛋白编号 | P06800 |
别名 | CD45, receptor-type tyrosine-protein phosphatase C, CD45R, L-CA, LY5, CD45 antigen, LCA, B220, PTPRC, protein tyrosine phosphatase, receptor type, C |
氨基酸序列 | Gln26Lys566 |
蛋白构建 | Gln26Lys566 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 120-160 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders. |
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CD45 Protein, Mouse, Recombinant (His) B-220 CD45 CD-45 receptor-type tyrosine-protein phosphatase C B 220 CD45R LY 5 CD 45 L-CA LY5 CD45 antigen LCA B220 LY-5 PTPRC protein tyrosine phosphatase, receptor type, C recombinant recombinant-proteins proteins protein