CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,510 | 5日内发货 | ||
500 μg | ¥ 10,000 | 5日内发货 | ||
1 mg | ¥ 16,800 | 5日内发货 |
生物活性 | Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. |
产品描述 | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | N-monomeric hFc |
蛋白编号 | P27548 |
别名 | IMD3, CD40LG, CD40L, IGM, TRAP, T-BAM, gp39, HIGM1, CD154, CD40LIGM, CD40 Ligand, TNFSF5, TNFSF5IMD3 |
蛋白构建 | Met112-Leu260 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 75.6 kDa (predicted). Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 200 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
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CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) HIGM-1 IMD 3 HIGM 1 CD 154 TNFSF 5 IMD3 CD40LG CD40L IGM TRAP T-BAM IMD-3 gp39 HIGM1 CD154 TNFSF-5 CD40LIGM CD-154 CD40 Ligand TNFSF5 TNFSF5IMD3 recombinant recombinant-proteins proteins protein