B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.1 kDa and the accession number is Q5ZPR3-2.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,360 | 5日内发货 | ||
500 μg | ¥ 9,490 | 5日内发货 | ||
1 mg | ¥ 15,800 | 5日内发货 |
生物活性 | Immobilized Human B7-H3, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.9ng/ml determined by ELISA. |
产品描述 | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.1 kDa and the accession number is Q5ZPR3-2. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | Q5ZPR3-2 |
别名 | B7H3, B7-H3, B7 homolog 3, PSEC0249, CD276, PRO352, UNQ309 |
蛋白构建 | Leu29-Pro245 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 50.1 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
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B7-H3 Protein, Human, Recombinant (aa 29-245, hFc) B7H3 PRO-352 B7-H3 B7 homolog 3 PRO 352 UNQ-309 CD 276 PSEC0249 PSEC 0249 UNQ309 CD276 PSEC-0249 PRO352 UNQ309 CD-276 UNQ 309 recombinant recombinant-proteins proteins protein