B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P42081-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,210 | 5日内发货 | ||
500 μg | ¥ 8,900 | 5日内发货 | ||
1 mg | ¥ 14,800 | 5日内发货 |
生物活性 | Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. |
产品描述 | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P42081-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P42081-1 |
别名 | B70, B7-2 antigen, B7-2, CD86 molecule, CD86, CD28LG2, MGC34413, BU63, FUN-1, B72, LAB72 |
蛋白构建 | Leu26-Pro247 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 28.2 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
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B7-2 Protein, Human, Recombinant (His & Avi) LAB-72 CD 86 FUN 1 CD-86 B-72 B70 B 72 B7-2 antigen B7-2 CD86 molecule MGC 34413 CD86 CD28LG2 MGC34413 BU63 B 70 BU-63 B-70 FUN1 FUN-1 B72 BU 63 LAB72 LAB 72 MGC-34413 recombinant recombinant-proteins proteins protein