B7-1 Protein, Human, Recombinant (His)

B7-1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.4 kDa and the accession number is A0N0P2.

规格	价格/CNY		货期	数量
100 μg	￥ 2,530	5日内发货
1 mg	￥ 20,700	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,530

5日内发货

1 mg

￥ 20,700

5日内发货

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) . The EC50 of of CTLA-4 (His & hFc Tag) is 35-80 ng/ml. 2.Loaded Recombinant Human CTLA-4 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human B7-1 Protein, His Tag with an affinity constant of 5.331nM as determined in BLI assay (Routinely tested). 3.Loaded Recombinant Human B7-1 Protein, His Tag on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag with an affinity constant of 0.46 µM as determined in BLI assay (QC tested). 4.Loaded Recombinant Human CD86 Protein, His Tag on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag with an affinity constant of 2.72 µM as determined in BLI assay (QC tested).

产品描述

B7-1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.4 kDa and the accession number is A0N0P2.

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	A0N0P2
别名	BB1, CD28LG1, B7-1, CD28LG, B7, B7.1, LAB7, CD80 molecule
蛋白构建	The extracellular domain (Met 1-Asn 242) of Human B7-1 (NP_005182.1) precursor was fused with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	25.4 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy