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Z-DEVD-FMK

产品编号 T6005 CAS 210344-95-9

别名: Caspase-3 Inhibitor

Z-DEVD-FMK (Caspase-3 Inhibitor) 是一种选择性、不可逆的 Caspase-3 抑制剂，IC50为 18 μM。它也抑制 caspase-6、caspase-7、caspase-8 和 caspase-10。

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Z-DEVD-FMK, CAS 210344-95-9

规格	价格/CNY	货期
1 mg	￥ 872	现货
5 mg	￥ 2,530	现货
10 mg	￥ 3,690	现货
25 mg	￥ 5,890	现货
50 mg	￥ 7,930	现货
100 mg	￥ 10,700	现货
1 mL * 10 mM (in DMSO)	￥ 3,250	现货

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选择批次

纯度: 99.69%

纯度: 96.19%

更多批次查询请联系客服

生物活性

化学信息

存储 & 溶解度

参考文献

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (139.1 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4955 mL	7.4776 mL	14.9553 mL	37.3882 mL
	5 mM	0.2991 mL	1.4955 mL	2.9911 mL	7.4776 mL
	10 mM	0.1496 mL	0.7478 mL	1.4955 mL	3.7388 mL
	20 mM	0.0748 mL	0.3739 mL	0.7478 mL	1.8694 mL
	50 mM	0.0299 mL	0.1496 mL	0.2991 mL	0.7478 mL
	100 mM	0.015 mL	0.0748 mL	0.1496 mL	0.3739 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Rickers A, et al. Eur J Immunol. 1998, (1), 296-304. 2. Yakovlev AG, et al. J Neurosci. 1997, 17(19), 7415-7424. 3. Wang J, et al. J Card Fail. 2000, 6(3), 243-249. 4. Meguro T, et al. Stroke. 2001, 32(2), 561-566. 5. Kanthasamy AG, et al. Free Radic Biol Med. 2006, 41(10), 1578-1589. 6. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons[J]. Neural Regeneration Research. 2020, 15(8): 1539. 7. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastin-induced ferroptosis in primary cortical neurons[J]. Neural regeneration research. 2020, 15(8): 1539. 8. Wang X Y, Wu K H, Pang H L, et al. Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)[J]. International journal of molecular sciences. 2019, 20(18): 4325. 9. Liu X, Song X, Luan D, et al. Real-time in situ Visualizing the Sequential Activation of Caspase Cascade Using a Multi-color Au-Se Bonding Fluorescent Nanoprobe[J]. Analytical chemistry. 2019 May 7;91(9):5994-6002. 10. Zhu Q, Ding L, Zi Z, et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis[J]. Cell reports. 2019 Mar 26;26(13):3657-3671.e5.

文献引用

1. Tang Q, Li W, Zheng X, et al. MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma. Signal Transduction and Targeted Therapy. 2020, 5(1): 1-12. 2. Zou Y, Zheng S, Xie X, et al. N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer. Nature Communications. 2022, 13(1): 1-18 3. Zhao Q, Feng H, Yang Z, et al. The central role of a two‐way positive feedback pathway in molecular targeted therapies‐mediated pyroptosis in anaplastic thyroid cancer. Clinical and Translational Medicine. 2022, 12(2): e727 4. Zhao Q, Feng H, Yang Z, et al. The central role of a two‐way positive feedback pathway in molecular targeted therapies‐mediated pyroptosis in anaplastic thyroid cancer. Clinical and Translational Medicine. 2022, 12(2): e727 5. Zhu Q, Ding L, Zi Z, et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Reports. 2019 Mar 26;26(13):3657-3671.e5 6. Liu X, Song X, Luan D, et al. Real-time in situ Visualizing the Sequential Activation of Caspase Cascade Using a Multi-color Au-Se Bonding Fluorescent Nanoprobe. Analytical Chemistry. 2019, 91(9): 5994-6002 7. Hussain M, Lu Y, Tariq M, et al. A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism. Iscience. 2022, 25(7): 104591. 8. Wang X Y, Wu K H, Pang H L, et al. Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera). International journal of molecular sciences. 2019, 20(18): 4325. 9. Xia H, Zhang Z, You F. Inhibiting ACSL1-Related Ferroptosis Restrains Murine Coronavirus Infection. Viruses. 2021, 13(12): 2383. 10. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons. Neural Regeneration Research. 2020, 15(8): 1539

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Z-DEVD-FMK 210344-95-9 Apoptosis Proteases/Proteasome Caspase ZDEVDFMK Inhibitor Z DEVD FMK Caspase-3 Inhibitor inhibit inhibitor

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

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产品描述	Z-DEVD-FMK (Caspase-3 Inhibitor) is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.
靶点活性	Caspase-3:18 μM.
体内活性	Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]
激酶实验	Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
细胞实验	N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

Z-DEVD-FMK

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords

别名	Caspase-3 Inhibitor
分子量	668.66
分子式	C30H41FN4O12
CAS No.	210344-95-9