store at low temperture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vinorelbine (KW-2307 base) 是一种半合成的长春花生物碱。它与微管蛋白结合并阻止有丝分裂纺锤体的形成,导致中期肿瘤细胞生长停滞。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 mg | ¥ 510 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 440 | 现货 |
产品描述 | Vinorelbine (KW-2307 base) is a semisynthetic vinca alkaloid. Vinorelbine binds to tubulin and prevents the formation of the mitotic spindle, resulting in the arrest of tumor cell growth in metaphase. |
靶点活性 | proliferation(Hela cells):1.25 nM |
体外活性 | Vinorelbine (0.5-5 nM) inhibits cell proliferation by 50% (IC50) at concentrations of 1.25 nM. At concentration of 8 nM vinorelbine, no cells are in anaphase[1]. Vinorelbine time-dependently induces the p53 and p21WAFI/CIP1 expression in androgen-dependent (AD) and- independent (AI) prostate cancer cell lines. Vinorelbine stimulates reporter genes in a concentration-dependent manner[2]. |
体内活性 | After vinorelbine treatment, the first neutropenicepisode occurred after the first (4 dogs), second (1), or sixth(1) vinorelbine treatment in the dogs[3]. Vinorelbine is tolerated at a weekly interval in tumor-bearing cats, with an MTD of 11.5 mg/m2[4]. |
激酶实验 | Reverse transcriptase assays: Reverse transcriptase assays are carried out in a reaction mixture (50 μL) containing 50 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM potassium chloride, 0.01% Triton X-100 or NP40, 10 μg/ml (dT)15.(A)n as template primer and [3H] deoxythymidine triphospahate. The reaction mixture is incubated for 1 hr at 37 ℃ and stopped by the addition of 50 μg of yeast tRNA and 2 mL of 10% solution of trichloroacetic acid containing 1 mM sodium pyrophosphate. The samples are filtered on filters (0.45 μm), washed first with 5% TCA solution for 5 times and then with 2 ml of 70% ethanol. The filters are dried, scintillation fluid is added and the radioactivity counted in a counter. |
别名 | 长春瑞宾, KW-2307 base |
分子量 | 778.93 |
分子式 | C45H54N4O8 |
CAS No. | 71486-22-1 |
store at low temperture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10 mg/mL (12.84 mM)
DMSO: 100 mg/mL (128.38 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 1.2838 mL | 6.4191 mL | 12.8381 mL | 32.0953 mL |
5 mM | 0.2568 mL | 1.2838 mL | 2.5676 mL | 6.4191 mL | |
10 mM | 0.1284 mL | 0.6419 mL | 1.2838 mL | 3.2095 mL | |
DMSO | 20 mM | 0.0642 mL | 0.321 mL | 0.6419 mL | 1.6048 mL |
50 mM | 0.0257 mL | 0.1284 mL | 0.2568 mL | 0.6419 mL | |
100 mM | 0.0128 mL | 0.0642 mL | 0.1284 mL | 0.321 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Vinorelbine 71486-22-1 Cytoskeletal Signaling Microtubule Associated 长春瑞宾 KW-2307 base Inhibitor inhibitor inhibit