Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TAI1 是一种具有口服活性的特异性 Hec1 抑制剂,有抗肿瘤活性,在 K562 细胞中的 GI50值为 13.48 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 295 | 现货 | ||
5 mg | ¥ 747 | 现货 | ||
10 mg | ¥ 1,230 | 现货 | ||
25 mg | ¥ 2,490 | 现货 | ||
50 mg | ¥ 3,490 | 现货 | ||
100 mg | ¥ 5,120 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 822 | 现货 |
产品描述 | TAI-1 is a potent and specific Hec1 inhibitor, which disrupts Hec1-Nek2 protein interaction. |
靶点活性 | K562 cells:13.48 nM (GI50) |
体外活性 | TAI-1 disrupts the binding of Nek2 to Hec1, which leads to degradation of Nek2 and chromosomal misalignment. TAI-1 shows strong growth inhibitory potency at nM levels across a broad spectrum of tumor cells, and produces synergistic activity with doxorubicin, topotecan and paclitaxel in leukemia, breast and liver cancer cells. [1] |
体内活性 | TAI-1 (20 mg/kg i.v. or 150 mg/kg p.o.) causes significant tumor growth delay in Huh-7 model and modest tumor inhibition in Colo205 and MDA-MB-231 models. [1] |
细胞实验 | Cells are seeded in 96 well plates, incubated for 24 hours, compounds added and incubated for 96 hours. All testing points are tested in triplicate wells. Cell viability is determined by MTS assay using CellTiter 96? Aqueous Non-radioactive Cell Proliferation Assay system according to manufacturer's instructions with MTS and PMS. Data retrieved from spectrophotometer are processed in Excel and GraphPad Prism 5 to calculate the concentration exhibiting 50% growth inhibition (GI50). All data represent the results of triplicate experiments.(Only for Reference) |
分子量 | 431.51 |
分子式 | C24H21N3O3S |
CAS No. | 1334921-03-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 80 mg/mL (185.4 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.3174 mL | 11.5872 mL | 23.1744 mL | 57.9361 mL |
5 mM | 0.4635 mL | 2.3174 mL | 4.6349 mL | 11.5872 mL | |
10 mM | 0.2317 mL | 1.1587 mL | 2.3174 mL | 5.7936 mL | |
20 mM | 0.1159 mL | 0.5794 mL | 1.1587 mL | 2.8968 mL | |
50 mM | 0.0463 mL | 0.2317 mL | 0.4635 mL | 1.1587 mL | |
100 mM | 0.0232 mL | 0.1159 mL | 0.2317 mL | 0.5794 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TAI-1 1334921-03-7 Apoptosis Cytoskeletal Signaling Microtubule Associated inhibit Inhibitor TAI 1 TAI1 inhibitor