首页工具

购物车

Stattic

产品编号 T6308 CAS 19983-44-9

别名: STAT3 Inhibitor V

Stattic (STAT3 Inhibitor V) 是一种 STAT3 抑制剂 (IC50=5.1 μM)，选择性地抑制 STAT3 活化、二聚化和核转位。Stattic 具有抗肿瘤活性，可诱导细胞凋亡。

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Stattic, CAS 19983-44-9

规格	价格/CNY	货期
5 mg	￥ 257	现货
10 mg	￥ 389	现货
25 mg	￥ 663	现货
50 mg	￥ 997	现货
100 mg	￥ 1,660	现货
200 mg	￥ 2,470	现货
500 mg	￥ 3,970	现货
1 mL * 10 mM (in DMSO)	￥ 389	现货

大包装超大折扣，点击咨询

选择批次

纯度: 99.76%

纯度: 99.64%

纯度: 98.78%

更多批次查询请联系客服

生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Stattic (STAT3 Inhibitor V) is a STAT3 inhibitor (IC50=5.1 μM) that selectively inhibits STAT3 activation, dimerization, and nuclear translocation. Stattic has antitumor activity and induces apoptosis.
靶点活性	STAT3:5.1 μM (cell free)
体外活性	方法：人胰腺癌细胞 PANC-1 和 BxPc-3 用 Stattic (1-10 μM) 处理 12-48 h，使用 CCK-8 方法检测细胞活力。结果：Stattic 以浓度和时间依赖的方式降低 PANC-1 和 BxPc-3 细胞增殖。Stattic 处理 24 h 后对 BxPc-3 和 PANC-1 细胞的 IC50 分别为 3.135-5.296 μM 和 3.835-4.165 μM。[1] 方法：人肝癌细胞 HepG2 用 Stattic (5-20 μM) 处理 1 h，随后用 IL-6 或 IFN-γ刺激，使用 Western Blot 方法检测靶点蛋白表达水平。结果：Stattic 的预孵育导致 STAT3 Tyr705 的磷酸化选择性降低，而 STAT1 Tyr701 的激活保持不变。[2]
体内活性	方法：为检测体内抗肿瘤活性，将 Stattic (10 mg/kg) 腹腔注射给携带人胰腺癌肿瘤 PANC-1 的 BALB/c nude 小鼠，每天一次，持续四周。结果：Stattic 通过灭活 STAT3 来抑制裸鼠肿瘤模型中的 PC 生长。[1] 方法：为研究在急性肝损伤中的作用，将 Stattic (5 mg/kg in DMSO:olive oil = 1:19) 单次腹腔注射给 LPS/d-GalN 诱导急性肝损伤的 BALB/c 小鼠。结果：Stattic 对 LPS/d-GalN 诱导的肝损伤具有保护作用，其保护作用可能与其抗炎和抗凋亡作用有关。[3]
激酶实验	The screening was performed at approximately 30C. The specificity of screening hits was validated in analogous assays for binding of the test compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays was 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides were: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY (PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. Peptides were >95% pure. For specificity analysis at 30°C, proteins were used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37°C, proteins were used at 370 nM (STAT3) or 100 nM (Lck). Proteins were incubated with test compounds in tubes at the indicated temperatures for 60 min prior addition of the respective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM). Analysis of c-Myc/Max and Jun/Jun dimerization and DNA binding at 37°C was performed as described but in the absence of DTT. Before measurement at room temperature, the mixtures were allowed to equilibrate for at least 30 min. Test compounds were used at the indicated concentrations diluted from 20× stock in DMSO. Binding curves and inhibition curves were fitted with SigmaPlot. All competition curves were repeated three times in independent experiments. For the analysis of time dependence of the inhibition, the components were mixed from stock solutions kept at 0C and then incubated at 37C. Aliquots were taken at the indicated time points [1].
细胞实验	MDA-MB-231, MDA-MB-435S, and MDA-MB-453 cells were seeded. at 5 × 10^4 cells in 6-well plates, grown for 24 hr before adding DMSO or Stattic (final DMSO concentration 0.1%) and then incubated with the inhibitor for 24 hr. All cells were collected and resuspended in buffer (0.1% sodium citrate, 0.1% Triton X-100, 20 μM propidium iodide) and incubated for 3 hr before 10^4 cells per sample were analyzed by flow cytometry with a FACSCalibur equipped with a 488 nm laser [1].

别名	STAT3 Inhibitor V
分子量	211.19
分子式	C8H5NO4S
CAS No.	19983-44-9

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: 1.1 mg/mL (5 mM)

DMSO: 10.6 mg/mL (50 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	4.7351 mL	23.6754 mL	47.3507 mL	118.3768 mL
Ethanol / DMSO	5 mM	0.947 mL	4.7351 mL	9.4701 mL	23.6754 mL
DMSO	10 mM	0.4735 mL	2.3675 mL	4.7351 mL	11.8377 mL
	20 mM	0.2368 mL	1.1838 mL	2.3675 mL	5.9188 mL
	50 mM	0.0947 mL	0.4735 mL	0.947 mL	2.3675 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Guo H, et al. Inhibition of STAT3Y705 phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells. Cell Death Discov. 2022 Mar 14;8(1):116. 2. Schust J, et al. Stattic: a small-molecule inhibitor of STAT3 activation and dimerization. Chem Biol. 2006 Nov;13(11):1235-42. 3. Li S, et al. Stattic alleviates acute hepatic damage induced by LPS/d-galactosamine in mice. Innate Immun. 2021 Feb;27(2):201-209. 4. Zhao C, Zhang B, Jiang J, et al. Up-regulation of ANXA1 suppresses polymorphonuclear neutrophil infiltration and myeloperoxidase activity by activating STAT3 signaling pathway in rat models of myocardial ischemia-reperfusion injury[J]. Cellular signalling. 2019, 62: 109325. 5. Lin I Y, Pan M H, Lai C S, et al. CCM111, the water extract of Antrodia cinnamomea, regulates immune-related activity through STAT3 and NF-κB pathways[J]. Scientific Reports. 2017 Jul 7;7(1):4862. 6. Ning T, Guo J, Zhang K, et al. Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway[J]. Stem cell research & therapy. 2019 Jan 24;10(1):45. 7. Chu K H, Lin S Y, Chiang B L. STAT6 Pathway Is Critical for the Induction and Function of Regulatory T Cells Induced by Mucosal B Cells[J]. Frontiers in immunology. 2020, 11.

文献引用

1. Wang X, Li X, Wang J, et al. SMGL-1/NBAS acts as a RAB-8 GEF to regulate unconventional protein secretion. Journal of Cell Biology. 2022, 221(7): e202111125 2. Li K, Fan L, Lin J, et al. Nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo. Science China Life Sciences. 2021: 1-13. 3. Zhang T, Wang Y, Li Q, et al. Mesenchymal stromal cells equipped by IFNα empower T cells with potent anti-tumor immunity. Oncogene. 2022: 1-16. 4. Zheng Q W, Ni Q Z, Zhu B, et al. PPDPF promotes lung adenocarcinoma progression via inhibiting apoptosis and NK cell-mediated cytotoxicity through STAT3. Oncogene. 2022: 1-13. 5. Chu K H, Lin S Y, Chiang B L. STAT6 Pathway Is Critical for the Induction and Function of Regulatory T Cells Induced by Mucosal B Cells. Frontiers in immunology. 2021 Jan 29;11:615868. doi: 10.3389/fimmu.2020.615868. eCollection 2020. 6. Ning T, Guo J, Zhang K, et al. Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway. Stem Cell Research & Therapy. 2019 Jan 24;10(1):45 7. Lin I Y, Pan M H, Lai C S, et al. CCM111, the water extract of Antrodia cinnamomea, regulates immune-related activity through STAT3 and NF-κB pathways. Scientific Reports. 2017, 7(1): 1-13 8. Zheng J M, Zhou H X, Yu H Y, et al. By Increasing the Expression and Activation of STAT3, Sustained C5a Stimulation Increases the Proliferation, Migration, and Invasion of RCC Cells and Promotes the Growth of Transgrafted Tumors. Cancer Management and Research. 2021, 13: 7607-7621. 9. Zhao C, Zhang B, Jiang J, et al. Up-regulation of ANXA1 suppresses polymorphonuclear neutrophil infiltration and myeloperoxidase activity by activating STAT3 signaling pathway in rat models of myocardial ischemia-reperfusion injury. Cellular Signalling. 2019, 62: 109325 10. He S, Lu M, Zhang L, et al.RSK4 promotes the macrophage recruitment and M2 polarization in esophageal squamous cell carcinoma.Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease.2023: 166996.

11. Yu L, Xu L, Chen Y, et al.IDO1 Inhibition Promotes Activation of Tumor-intrinsic STAT3 Pathway and Induces Adverse Tumor-protective Effects.The Journal of Immunology.2024 12. Liu H, Sun L, Zhao H, et al.Proteinase 3 depletion attenuates leukemia by promoting myeloid differentiation.Cell Death & Differentiation.2024: 1-14.

下拉查看更多收起

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Stattic 19983-44-9 Apoptosis JAK/STAT signaling Stem Cells STAT inhibit Alport PC3M-1E8 cancer p-STAT3 prostate S727 syndrome Inhibitor P-ERK1/2 SH2 STAT3 Inhibitor V S phase Y705 inhibitor

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

沪公网安备 31010602006700号

陶术

生物

TargetMol^®中国区唯一合作伙伴

点击进入陶术生物官网

联系我们

电话：

400-820-0310

邮箱：

service@tsbiochem.com

QQ：

2881945090（终端用户）

2885109491（经销商）

经销：

终端：

地址：

上海市静安区江场三路238号8楼

Stattic

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords