首页 工具
登录
购物车
Sabutoclax

Sabutoclax

产品编号 T6650   CAS 1228108-65-3
别名: BI-97C1

Sabutoclax (BI-97C1) 是一种泛 Bcl-2 抑制剂,包括 Bcl-xL、Bcl-2、Mcl-1 和 Bfl-1,IC50 分别为 0.31 μM、0.32 μM、0.20 μM 和 0.62 μM。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
Sabutoclax Chemical Structure
Sabutoclax, CAS 1228108-65-3
规格 价格/CNY 货期 数量
1 mg ¥ 575 现货
5 mg ¥ 1,390 现货
10 mg ¥ 2,230 现货
25 mg ¥ 4,490 现货
50 mg ¥ 6,570 现货
100 mg ¥ 8,860 现货
1 mL * 10 mM (in DMSO) ¥ 1,550 现货
产品目录号及名称: Sabutoclax (T6650)
点击图片重新获取验证码
选择批次  
纯度: 98.5%
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Sabutoclax (BI-97C1)(BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.
靶点活性 Bcl-xL:0.31 μM, Bfl-1:0.62 μM, BCL2:0.32 μM, Mcl-1:0.2 μM
体外活性 Sabutoclax (0.001-10 μM; 72 hours) inhibits the growth of human prostate cancer and lung cancer cell lines[2].
Sabutoclax (0.01-1 μM; 24-48 hours) induces apoptosis in human diffuse large B-cell lymphoma cells[2].
Sabutoclax (0-15 μM; 48 hours) upregulates the levels of pro-apoptotic proteins in chemotherapy-resistant cells[1].
体内活性 Sabutoclax (1-5 mg/kg; intraperitoneal injection; every two days for a total of 18 days) reduces the growth of M2182 tumors in nude mice[2].
激酶实验 Competitive fluorescence polarization assays (FPA) : A Bak BH3 peptide (F-BakBH3) (GQVGRQLAIIGDDINR) is labeled at the N-terminus with fluorescein isothiocyanate (FITC) and purified by HPLC. For competitive binding assays, 100 nM GST-Bcl-XL ΔTM protein is preincubated with the tested compound at varying concentrations in 47.5 μL PBS (pH = 7.4) in 96-well black plates at room temperature for 10 min, and then 2.5 μL of 100 nM FITC-labeled Bak BH3 peptide is added to produce a final volume of 50 μL. The wild-type and mutant Bak BH3 peptides are included in each assay plate as positive and negative controls, respectively. After 30 min incubation at room temperature, the polarization values in millipolarization units are measured at excitation/emission wavelengths of 480/535 nm with a multilabel plate reader. IC50 is determined by fitting the experimental data to a sigmoidal dose-response nonlinear regression model. Data reported are mean of three independent experiments. Performance of Bcl-2 and Mcl-1FPA are similar. Briefly, 50 nM of GST-Bcl-2 or -Mcl-1are incubatedwith various concentrations of compound (4 and 11-14) for 2 min, and then 15 nM FITC-conjugated-Bim BH3 peptide is added in PBS buffer. Fluorescence polarization is measured after 10 min.
细胞实验 ATP-LITE assay(Only for Reference)
别名 BI-97C1
分子量 700.78
分子式 C42H40N2O8
CAS No. 1228108-65-3

存储

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 22 mg/mL(31.4 mM)

DMSO: 93 mg/mL (132.7 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 1.427 mL 7.1349 mL 14.2698 mL 35.6745 mL
5 mM 0.2854 mL 1.427 mL 2.854 mL 7.1349 mL
10 mM 0.1427 mL 0.7135 mL 1.427 mL 3.5675 mL
20 mM 0.0713 mL 0.3567 mL 0.7135 mL 1.7837 mL
DMSO 50 mM 0.0285 mL 0.1427 mL 0.2854 mL 0.7135 mL
100 mM 0.0143 mL 0.0713 mL 0.1427 mL 0.3567 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Yunhui Hu, et Al. Sabutoclax, pan-active BCL-2 protein family antagonist, overcomes drug resistance and eliminates cancer stem cells in breast cancer. Cancer Lett. 2018 Jun 1:423:47-59. 2. Wei J , et al. BI-97C1, an optically pure Apogossypol derivative as pan-active inhibitor of antiapoptotic B-cell lymphoma/leukemia-2 (Bcl-2) family proteins. J Med Chem. 2010 May 27; 53(10):4166-76.
Paris saponin VII Rasagiline Myristicin ABT-737 BTSA1 BM 957 A-385358 Obovatol

相关化合物库

该产品包含在如下化合物库中:
抗癌活性化合物库 抑制剂库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Sabutoclax 1228108-65-3 Apoptosis BCL BI-97C1 Inhibitor inhibitor inhibit

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼