Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Roxithromycin (RU-28965) 是半合成的大环内酯类抗生素。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 g | ¥ 374 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 315 | 现货 |
产品描述 | Roxithromycin (RU-28965) is a semi-synthetic derivative of the macrolide antibiotic erythromycin with antibacterial and anti-malarial activities. |
激酶实验 | The half-maximal inhibitory concentrations (IC50s) of Fenofibrate, statins (atorvastatin, lovastatin, pravastatin, simvastatin and simvastatin acid, the active form of simvastatin) and glipizide for recombinant human CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 are determined using fluorometric CYP450 inhibition assays. Briefly, the drugs are dissolved in methanol or acetonitrile. In 96 well assay plates, the drugs are diluted to a series of concentrations in a solution containing cofactors including NADP+ (final concentration 1.3 mM), MgCl2 (final concentration 3.3 m M), glucose-6-phosphate (G6P, final concentration 3.3 mM) and glucose 6-phosphate dehydrogenase (final concentration 0.4 U/mL). The mixture is pre-incubated at 37°C for 10 min. The enzymes and fluorogenic substrates are diluted to desired concentrations in sodium phosphate reaction buffer (pH 7.4, final concentration 200 mM) and mixed. Reactions are initiated with addition of the enzyme and substrate mixture to the cofactor and drug mixture. The final reaction volume of all assays is 200 μL. After incubating at 37°C for a pre-specified period of time (15 to 45 min), the reactions are stopped with addition of 75 μL quenching solution (0.5 M Tris base or 2N NaOH). Fluorescence is determined using a BioTek Synergy 2 fluorescence reader. Each of the drugs is tested at eight concentrations in duplicate. To estimate IC50s, percent of inhibition is calculated using net fluorescence that is corrected for the background. The values of percent of inhibition are then fitted to a three or four parameter log-logistic model[1]. |
别名 | Roxl-150, 罗红霉素, RU-28965 |
分子量 | 837.05 |
分子式 | C41H76N2O15 |
CAS No. | 80214-83-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 154 mg/mL (184 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 154 mg/mL (184 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.1947 mL | 5.9734 mL | 11.9467 mL | 29.8668 mL |
5 mM | 0.2389 mL | 1.1947 mL | 2.3893 mL | 5.9734 mL | |
10 mM | 0.1195 mL | 0.5973 mL | 1.1947 mL | 2.9867 mL | |
20 mM | 0.0597 mL | 0.2987 mL | 0.5973 mL | 1.4933 mL | |
50 mM | 0.0239 mL | 0.1195 mL | 0.2389 mL | 0.5973 mL | |
100 mM | 0.0119 mL | 0.0597 mL | 0.1195 mL | 0.2987 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Roxithromycin 80214-83-1 Microbiology/Virology ribosome Antibacterial Antibiotic Roxl-150 罗红霉素 RU 28965 Inhibitor RU-28965 Bacterial inhibit RU28965 inhibitor