Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RI-1 (RAD51 inhibitor 1) 是一种选择性 RAD51的抑制剂,IC50值为 5-30 μM,可以破坏人类细胞中的同源重组。它在半胱氨酸 319 处与 RAD51 蛋白表面共价结合,并通过直接结合到 RAD51 细丝中蛋白质亚基之间的界面的蛋白质表面来灭活 RAD51。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 266 | 现货 | ||
2 mg | ¥ 378 | 现货 | ||
5 mg | ¥ 622 | 现货 | ||
10 mg | ¥ 1,080 | 现货 | ||
25 mg | ¥ 1,950 | 现货 | ||
50 mg | ¥ 3,730 | 现货 | ||
100 mg | ¥ 5,450 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 683 | 现货 |
产品描述 | RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM). |
靶点活性 | RAD51:<30 μM |
体内活性 | 在HeLa细胞,MCF-7和U2OS三种癌细胞株中,RI-1具有单剂毒性(LD50:20-40 μM)。 通过直接和特异破坏HsRAD51和抑制RAD51,RI-1可抑制RAD51在ssDNA上形成丝的能力,从而使细胞对DNA损伤的敏感性增加。RI-1使G2期细胞的γ-H2AX灶减少,并使辐射6小时后未修复DSB的水平增加。 |
激酶实验 | DNA binding assays: All reactions are performed in black non-binding polystyrene 384-well plates with reaction volumes of 30–100 μL. Purified DNA strand exchange proteins and chemical compounds are pre-incubated at room temperature for 5 minutes; they are then further incubated at 37°C for 30 min with 100 nM of ssDNA substrate, consisting of a 45-mer poly-dT tagged with Alexa 488 at the 5' terminus (synthesized and purified by Integrated DNA Technologies). Reactions are performed in 20 mM HEPES pH 7.5, 10 mM MgCl2, 0.25 μM BSA, 2% glycerol, 30 mM NaCl, 4% DMSO and 2 mM ATP. Some conditions included DTT or TCEP (tris(2-carboxyethyl)phosphine) as indicated. DNA binding is measured as a function of fluorescence polarization (FP) with a Safire2 plate reader, using the following settings: excitation 470±5 nM, emission 530±5 nM, 10 reads/well, Z height and G factor auto-calibrated from control wells. Displayed error bars represent standard deviation. For experiments involving a titration of protein concentrations, data are fit to an equation that accounts for the cooperative nature by which recombinase proteins bind DNA. For experiments involving a titration of RI-1, protein concentrations are selected to give an ~80% saturation of the FP signal in the absence of RI-1. |
细胞实验 | Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference) |
别名 | RI-1; 3-氯-1-(3,4-二氯苯基)-4-(4-吗啉基)-1H-吡咯-2,5-二酮, RI1, RAD51 inhibitor 1 |
分子量 | 361.61 |
分子式 | C14H11Cl3N2O3 |
CAS No. | 415713-60-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 36.2 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7654 mL | 13.8271 mL | 27.6541 mL | 69.1353 mL |
5 mM | 0.5531 mL | 2.7654 mL | 5.5308 mL | 13.8271 mL | |
10 mM | 0.2765 mL | 1.3827 mL | 2.7654 mL | 6.9135 mL | |
20 mM | 0.1383 mL | 0.6914 mL | 1.3827 mL | 3.4568 mL | |
50 mM | 0.0553 mL | 0.2765 mL | 0.5531 mL | 1.3827 mL | |
100 mM | 0.0277 mL | 0.1383 mL | 0.2765 mL | 0.6914 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RI-1 415713-60-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis recombination RI-1; 3-氯-1-(3,4-二氯苯基)-4-(4-吗啉基)-1H-吡咯-2,5-二酮 RI1 homologous RI 1 Inhibitor RAD51 RAD51 inhibitor 1 inhibit inhibitor