store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Palmitoylethanolamide (Impulsin) 是一种内源性脂肪酸酰胺。它有消炎药、降压药、神经保护剂和抗惊厥药的作用。在给药后,Palmitoylethanolamide 可抑制促炎介质从活化肥大细胞的释放。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 282 | 现货 | ||
25 mg | ¥ 456 | 现货 | ||
50 mg | ¥ 663 | 现货 | ||
100 mg | ¥ 1,060 | 现货 | ||
200 mg | ¥ 1,570 | 现货 | ||
500 mg | ¥ 2,660 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 198 | 现货 |
产品描述 | Palmitoylethanolamide (Impulsin) is an endogenously fatty acid amide. It has a role as an anti-inflammatory drug, an antihypertensive agent, a neuroprotective agent and an anticonvulsant.Upon administration, palmitoylethanolamide may inhibit the release of pro-inflammatory mediators from activated mast cells. |
靶点活性 | PPARα:3.1±0.4 μM(EC50) |
体外活性 | PEA protects cultured mouse cerebellar granule cells from glutamate toxicity and enhances microglial cell motility. In the mitochondrial fraction from cells stimulated with PEA, steroidogenic acute regulatory protein (StAR) and cytochrome P450 enzyme(P450scc) expression increases, both comprising proteins considered to be involved in crucial steps of neurosteroid formation. Moreover, PEA shows a protective effect, reducing malondialdehyde formation in cells treated with L-buthionine-(S,R)-sulfoximine, a glutathione depletor and the effect of PEA is partially inhibited by finasteride, a 5a-reductase inhibitor[2]. |
体内活性 | PEA attenuates inflammation in wild-type mice but has no effect in mice deficient in PPAR-α. PEA has been shown to inhibit peripheral inflammation and mast-cell degranulation as well as to exert neuroprotective and antinociceptive effects in rats and mice. These actions are accompanied by changes in nitric oxide production, neutrophil influx, and expression of proinflammatory proteins such as inducible nitric oxide synthase and cyclooxygenase-2[1]. In addition to its known anti-inflammatory activity, PEA regulates many pathophysiological processes, including pain perception, convulsions, and neurotoxicity. In the central nervous system (CNS), where PEA is present at detectable levels, its concentrations significantly increase under pathological conditions, such as excitotoxicity, brain ischaemia and neuroinflammation[2]. |
细胞实验 | C6 glioma cells (300 000?P60 dish) or astrocyte primary culture are incubated in serum-free DMEM at 37℃ for at least 24 h before each experiment. Then, cells are treated with PEA (10 μM) for 24 h in serum-free medium, in the presence and absence of GW6471 (10 μM) added 30 min before ethanolamide treatment. The concentration of PEA is chosen on the basis of preliminary experiments, assessing drug efficacy without modifi-cation of cell viability. PEA and GW6471 are first dissolved in absolute ethanol and then diluted with DMEM. The final ethanol concentration was< 0.5%. Mitochondrial protein extracts from C6 or astrocytes are obtained. Alternatively after 24 h of starvation in serum-free DMEM, C6 cells are treated with FIN (100 nM). After 30 min, PEA (10 μM) and?or ALLO (3 μM) is added and, 30 min later, are stimulated with BSO (10 mM). After 18 h, MDA is evaluated. In this set of experiments, FIN and?or PEA are dissolved in dimethylsulphoxide (DMSO) and then diluted with DMEM (0.1% final DMSO concentration).(Only for Reference) |
别名 | Mackpeart DR 14V, 帕米醇, N-palmitoylethanolamine, Loramine P 256, Impulsin, AM 3112, Palmidrol |
分子量 | 299.49 |
分子式 | C18H37NO2 |
CAS No. | 544-31-0 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6 mg/mL (20 mM)
Ethanol: 7.5 mg/mL (25 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.339 mL | 16.695 mL | 33.3901 mL | 83.4752 mL |
5 mM | 0.6678 mL | 3.339 mL | 6.678 mL | 16.695 mL | |
10 mM | 0.3339 mL | 1.6695 mL | 3.339 mL | 8.3475 mL | |
20 mM | 0.167 mL | 0.8348 mL | 1.6695 mL | 4.1738 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Palmitoylethanolamide 544-31-0 DNA Damage/DNA Repair Metabolism Microbiology/Virology Influenza Virus Endogenous Metabolite PPAR inhibit Loramine P-256 Mackpeart DR 14V 帕米醇 N-palmitoylethanolamine AM-3112 Loramine P 256 Inhibitor Loramine P256 AM3112 Impulsin AM 3112 Palmidrol inhibitor