Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PQ401 (IGF-1R Inhibitor II) 是一种IGF-IR 抑制剂。在 MCF-7 细胞中,它抑制 IGF-I 刺激的 IGF-IR 自磷酸化,可有效抑制 IGF-I 刺激的 MCF-7 细胞生长。它诱导 caspase 介导的细胞凋亡,有用于乳腺癌和其他对 IGF-I 敏感的癌症的研究潜力。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 198 | 现货 | ||
5 mg | ¥ 498 | 现货 | ||
10 mg | ¥ 883 | 现货 | ||
25 mg | ¥ 1,650 | 现货 | ||
50 mg | ¥ 2,790 | 现货 | ||
100 mg | ¥ 4,180 | 现货 | ||
500 mg | ¥ 8,820 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 557 | 现货 |
产品描述 | PQ401 (IGF-1R Inhibitor II) suppresses autophosphorylation of IGF-1R domain(IC50<1 μM). |
靶点活性 | IGF-1R:<1 μM |
体内活性 | Treated mice received 50 or 100 mg/kg PQ401 prepared in 8% polysorbate 80/ethanol/PBS, administered i.p. thrice a week. |
激酶实验 | IGF-IR Peptide Autophosphorylation: One microgram of constitutively active IGF-IR kinase domain peptide isincubated +/? varying concentrations of PQ 401 in 2% DMSO in 40 mM Tris (pH 7.4), 80 μMEGTA, 0.25% 2-mercaptoethanol, 80 μM Na3VO4, 10 mM MgCl2, and 2 mM MnCl2 for 20 minutes. ATP isthen added at a final concentration of 20 μM. Autophosphorylation of the kinase domain peptide isallowed to occur for 20 minutes at 22℃. The reaction isstopped by the addition of SDS-reducing buffer and the samples are run on SDS-PAGE. Following transfer to nitrocellulose membrane, peptide autophosphorylation isdetermined by Western blotting employing an antibody against phosphotyrosine (PY20). |
细胞实验 | The inhibitory effects of diaryl urea on breast cancer cell growth are determined using a CyQuant cell proliferation assay kit. MCF-7 or MCNeuA cells are plated in 96-well plates (5 × 103 per well) in phenol red-free DMEM supplemented with 10% FCS. One plate isprepared for each harvest day. Cells are allowed to adhere overnight and are then treated with various concentrations of diaryl urea or DMSO as a vehicle control. Microplate cultures are harvested on days 0, 1, 2, and 3 by inverting the microplate onto paper towels with gentle blotting to remove growth medium without disrupting adherent cells. Each plate iskept at ?80 ℃ until the end of the experiment (day 3) when all of the plates are thawed and assayed together. After thawing, 200 μL of CyQuant GR solution are added to each well and the plates are incubated in the dark for 2 to 5 minutes. Fluorescence ismeasured with a SpectraMax Gemini XS fluorescence microplate reader with 480-nm excitation and 520-nm emission. Proliferation index iscalculated as the percent of nucleotide content versus control cells at day 0.(Only for Reference) |
别名 | IGF-1R Inhibitor II |
分子量 | 341.79 |
分子式 | C18H16ClN3O2 |
CAS No. | 196868-63-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 8.5 mg/mL (25 mM)
DMSO: 17.1 mg/mL (50 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.9258 mL | 14.6289 mL | 29.2577 mL | 73.1443 mL |
5 mM | 0.5852 mL | 2.9258 mL | 5.8515 mL | 14.6289 mL | |
10 mM | 0.2926 mL | 1.4629 mL | 2.9258 mL | 7.3144 mL | |
20 mM | 0.1463 mL | 0.7314 mL | 1.4629 mL | 3.6572 mL | |
DMSO | 50 mM | 0.0585 mL | 0.2926 mL | 0.5852 mL | 1.4629 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PQ401 196868-63-0 Apoptosis Tyrosine Kinase/Adaptors IGF-1R MCF-7 cells breast inhibit caspase PQ-401 PQ 401 autophosphorylation IGF-1R Inhibitor II cancer Inhibitor inhibitor