Powder: -20°C for 3 years | In solvent: -80°C for 1 year
OG-L002 是一种高度选择性的 LSD1有效抑制剂,IC50为 0.02 μM。它抑制HSV IE 基因的表达,还是单胺氧化酶抑制剂,对 MAO-A 和 MAO-B 的IC50分别为 1.38 μM 和 0.72 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 311 | 现货 | ||
2 mg | ¥ 459 | 现货 | ||
5 mg | ¥ 828 | 现货 | ||
10 mg | ¥ 1,390 | 现货 | ||
25 mg | ¥ 2,580 | 现货 | ||
50 mg | ¥ 3,850 | 现货 | ||
100 mg | ¥ 5,520 | 现货 | ||
500 mg | ¥ 11,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 513 | 现货 |
产品描述 | OG-L002 is an effective and selective LSD1 inhibitor (IC50: 20 nM), showing 69- and 36-fold selectivity over MAO-A and MAO-B, respectively. |
靶点活性 | LSD1:20 nM |
体外活性 | OG-L002 potently inhibits HSV IE gene expression in both HeLa and HFF cells with IC50 of ~10 μM and ~3 μM , respectively. OG-L002 treatment (50 μM ) results in the reduced production of progeny virus with no significant toxicity in HeLa or HFF cells. OG-L002 (50 μM ) increases the levels of repressive chromatin on viral IE gene promoters. In addition, OG-L002 also represses the expression of hCMV IE genes and adenovirus E1A gene. [1] |
体内活性 | OG-L002 (6 to 40 mg/kg) represses HSV primary infection in a dose-dependent manner in a mouse model. Moreover, OG-L002 also represses HSV reactivation from latency in a mouse ganglion explant model. [1] |
激酶实验 | LSD1 demethylation assay: Human recombinant LSD1 protein is incubated with dimethylated H3K4 peptide as the substrate in the presence of various concentrations of lead compound inhibitors (0 to 75 μM ) or control tranylcypromine. The demethylase activity is measured by the release of Water2 produced during the catalytic process, using the Amplex red peroxide/peroxidase-coupled assay kit. Each reaction is done in triplicate. The maximum LSD1 demethylase activity is obtained in the absence of inhibitor and corrected for background fluorescence. The Ki (IC50) of OG-L002 is calculated as half-maximal activity. |
细胞实验 | HeLa or HFF cells are treated with the indicated concentrations of saponin (positive control) or compound OG-L002 for 12 hours. Cytotoxicity is determined using conditions recommended by the manufacturer and expressed as ratios to the cytotoxicity of the DMSO control.(Only for Reference) |
分子量 | 225.29 |
分子式 | C15H15NO |
CAS No. | 1357302-64-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 16 mg/mL (71 mM)
DMSO: 42 mg/mL (186.4 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 4.4387 mL | 22.1936 mL | 44.3872 mL | 110.9681 mL |
5 mM | 0.8877 mL | 4.4387 mL | 8.8774 mL | 22.1936 mL | |
10 mM | 0.4439 mL | 2.2194 mL | 4.4387 mL | 11.0968 mL | |
20 mM | 0.2219 mL | 1.1097 mL | 2.2194 mL | 5.5484 mL | |
50 mM | 0.0888 mL | 0.4439 mL | 0.8877 mL | 2.2194 mL | |
DMSO | 100 mM | 0.0444 mL | 0.2219 mL | 0.4439 mL | 1.1097 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
OG-L002 1357302-64-7 Chromatin/Epigenetic Microbiology/Virology Neuroscience Histone Demethylase Monoamine Oxidase HSV Inhibitor MAO-B LSD1 oxidases MAO inhibit monoamine Herpes simplex virus OGL002 MAO-A OG L002 OG-L-002 OG-L 002 inhibitor