Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NS11394 是可口服GABAA 的阳性变构调节剂 (PAM),Ki 值为 0.5 nM。它对GABAA 的选择性依次为 α5、α3、α2 和α1 受体,具有抗炎和抗焦虑活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 329 | 现货 | ||
2 mg | ¥ 478 | 现货 | ||
5 mg | ¥ 779 | 现货 | ||
10 mg | ¥ 1,230 | 现货 | ||
25 mg | ¥ 2,260 | 现货 | ||
50 mg | ¥ 3,730 | 现货 | ||
100 mg | ¥ 5,430 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 997 | 现货 |
产品描述 | NS11394 is a effective and subtype-selective GABA(A) receptor-positive modulator; possesses a functional efficacy selectivity profile of α(5) > α(3) > α(2) > α(1) at GABA(A) alpha subunit-containing receptors. |
激酶实验 | Inhibition of FLT3 autophosphorylation: To measure inhibition of FLT3 autophosphorylation, MV4-11 or RS4;11 cells are cultured in low serum media (0.5% FBS) overnight and seeded at a density of 400 000 cells per well in a 96-well plate the following day. The cells are incubated with different concentrations of AC220 for 2 hours at 37 °C. To induce FLT3 autophosphorylation in RS4;11 cells, 100 ng/mL FLT3 ligand is added for 15 minutes after the 2-hour AC220 incubation. Cell lysates are prepared and incubated in 96-well plates precoated with a total FLT3 capture antibody. The coated plates are incubated with either a biotinylated antibody against FLT3 to detect total FLT3 or an antibody against phosphotyrosines to detect FLT3 autophosphorylation. In both cases, a SULFO-tagged streptavidin secondary antibody is used for electrochemiluminescence detection on the Meso Scale Discovery platform. The concentration of AC220 that inhibits FLT3-ITD or TLT3-WT autophosphorylation by 50% represents IC50 value |
别名 | NS 11394 |
分子量 | 353.42 |
分子式 | C23H19N3O |
CAS No. | 951650-22-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (169.77 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.8295 mL | 14.1475 mL | 28.2949 mL | 70.7374 mL |
5 mM | 0.5659 mL | 2.8295 mL | 5.659 mL | 14.1475 mL | |
10 mM | 0.2829 mL | 1.4147 mL | 2.8295 mL | 7.0737 mL | |
20 mM | 0.1415 mL | 0.7074 mL | 1.4147 mL | 3.5369 mL | |
50 mM | 0.0566 mL | 0.2829 mL | 0.5659 mL | 1.4147 mL | |
100 mM | 0.0283 mL | 0.1415 mL | 0.2829 mL | 0.7074 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NS11394 951650-22-9 Membrane transporter/Ion channel Neuroscience GABA Receptor Inhibitor Anxiety cord γ-Aminobutyric acid Receptor neuropathi electrophysiological Freund’s inhibit Complete pain NS 11394 Gamma-aminobutyric acid Receptor adjuvant inflammatory pinal NS-11394 inhibitor