Powder: -20°C for 3 years | In solvent: -80°C for 1 year
LB-100 是一种新型蛋白磷酸酶 2A (PP2A) 抑制剂,可使恶性脑膜瘤细胞对放射治疗效果敏感。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 412 | 现货 | ||
2 mg | ¥ 598 | 现货 | ||
5 mg | ¥ 995 | 现货 | ||
10 mg | ¥ 1,560 | 现货 | ||
25 mg | ¥ 3,230 | 现货 | ||
50 mg | ¥ 4,690 | 现货 | ||
100 mg | ¥ 6,780 | 现货 | ||
500 mg | ¥ 13,500 | 现货 |
产品描述 | LB-100, a novel Protein Phosphatase 2A (PP2A) inhibitor, sensitizes malignant meningioma cells to the therapeutic effects of radiation |
体外活性 | LB-100, a small molecular inhibitor of protein phosphatase 2A (PP2A), has been shown to enhance T cell anti-tumor activity through activation of the mTOR signaling pathway.?Herein, Investigated if a treatment strategy consisting of a combination of LB-100 and anti-CAIX CAR-T cell therapy produced a synergistic anti-tumor effect.?LB-100 enhanced anti-CAIX CAR-T cell treatment efficacy in vitro and in vivo.LB-100 in augmenting the cytotoxic activity of anti-CAIX CAR-T cells and underscore the synergistic therapeutic potential of applying combination LB-100 and CAR-T Cell therapy to other solid tumors[1]. |
激酶实验 | PP2A activity assays: Cultured pancreatic cancer cells are treated with IC50 of LB-100 for each cell line or equal volume of vehicle for 2 hours, and PP2A activity assays are then performed using Ser/Thr phosphatase assay kit. Cells are lysed with an ultrasonic cell disruptor, and the PP2A concentration is measured using a Ser/Thr phosphatase assay kit according to the instructions. Assays for each cell line are performed in triplicate. |
细胞实验 | Cytotoxicity is conducted by using a Cell Counting Kit-8. Cells are seeded in 96-well plates with a density of 3000 cells per well and are assessed after treatments following the CCK-8 protocol. Relative cytotoxicity is expressed as a percentage of speci?c controls. (Only for Reference) |
分子量 | 268.31 |
分子式 | C13H20N2O4 |
CAS No. | 2061038-65-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: < 1 mg/mL (insoluble or slightly soluble)
H2O: 49 mg/mL (182.6 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 3.727 mL | 18.6352 mL | 37.2703 mL | 93.1758 mL |
5 mM | 0.7454 mL | 3.727 mL | 7.4541 mL | 18.6352 mL | |
10 mM | 0.3727 mL | 1.8635 mL | 3.727 mL | 9.3176 mL | |
20 mM | 0.1864 mL | 0.9318 mL | 1.8635 mL | 4.6588 mL | |
50 mM | 0.0745 mL | 0.3727 mL | 0.7454 mL | 1.8635 mL | |
100 mM | 0.0373 mL | 0.1864 mL | 0.3727 mL | 0.9318 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(Rac)-LB-100 2061038-65-9 Metabolism Phosphatase LB100 LB-100 LB 100 Inhibitor inhibitor inhibit