Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Gimeracil (Gimestat) 是一种竞争性可逆的 DPYD(二氢嘧啶脱氢酶,DPD) 有效抑制剂,是口服氟嘧啶衍生物 S-1 的成分,能够抑制 DNA DSB 的修复。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 297 | 现货 | ||
25 mg | ¥ 460 | 现货 | ||
50 mg | ¥ 646 | 现货 | ||
100 mg | ¥ 832 | 现货 | ||
500 mg | ¥ 1,263 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 494 | 现货 |
产品描述 | Gimeracil (Gimestat) is a competitive, reversible inhibitor of dihydropyrimidine dehydrogenase. |
激酶实验 | Microdilution method: The culture media used are RPMI 1640 with glutamine, without bicarbonate and phenol red, buffered with morpholinopropanesulfonic acid (MOPS) (0.165 M, pH 7.0). Two-fold serial dilutions of Flucytosine (0.06-64 μg/mL) are prepared and dispensed in 50 uL aliquot, in flat-bottom 96-well assay plates which are kept frozen at -70 °C in sealed plastic bags until used. The inoculum is prepared spectrophotometrically and standardized to a concentration of 1.0-5.0 × 103 cfu per mL. A 50 μL volume of this suspension is used to inoculate each well containing 50 μL of the double concentration of Flucytosine to be tested. Once inoculated, each well therefore contains 100 μL of broth favoured over 200 μL to facilitate the agitation of the plates prior to spectrophotometric reading. After an incubation period of 24 and 48 hours at 35 °C, the plates are agitated for 3 minutes at 900 r.p.m. with a shaker and the optical density of the growth in each well is determined with the use of an automatic plate reader set at 495 nm. The inhibitory concentration of IC50 is computed mathematically. |
别名 | 吉美嘧啶, 吉莫斯特, Gimestat |
分子量 | 145.54 |
分子式 | C5H4ClNO2 |
CAS No. | 103766-25-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 28 mg/mL (192.4 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 6.871 mL | 34.3548 mL | 68.7096 mL | 171.7741 mL |
5 mM | 1.3742 mL | 6.871 mL | 13.7419 mL | 34.3548 mL | |
10 mM | 0.6871 mL | 3.4355 mL | 6.871 mL | 17.1774 mL | |
20 mM | 0.3435 mL | 1.7177 mL | 3.4355 mL | 8.5887 mL | |
50 mM | 0.1374 mL | 0.6871 mL | 1.3742 mL | 3.4355 mL | |
100 mM | 0.0687 mL | 0.3435 mL | 0.6871 mL | 1.7177 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Gimeracil 103766-25-2 Autophagy 吉美嘧啶 吉莫斯特 Lu-99 inhibit Inhibitor Gimestat LC-11 repair DNA PAN-4 KB/C3 radiotherapy inhibitor