Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GSK0660 是 PPARβ/δ 的拮抗剂和反向激动剂,IC50均为155 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 295 | 现货 | ||
2 mg | ¥ 418 | 现货 | ||
5 mg | ¥ 672 | 现货 | ||
10 mg | ¥ 990 | 现货 | ||
25 mg | ¥ 1,970 | 现货 | ||
50 mg | ¥ 3,660 | 现货 | ||
100 mg | ¥ 5,330 | 现货 | ||
500 mg | ¥ 10,900 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 739 | 现货 |
产品描述 | GSK0660 is an antagonist and inverse agonist of PPARβ/δ. |
靶点活性 | PPAR-β/δ:155 nM |
激酶实验 | Human astrocytoma cells, 1321N1, are grown to stably express rat P2X7, human P2X4, P2X2a, P2X2/3, P2X1, P2Y1?and P2Y2 recombinant receptors. Agonist, BzATP, 2,3-O-(4-ben-zoylbenzoyl)-ATP or ATP-induced changes in intracellular Ca2+?concentrations are assessed in all of the cell lines using the Ca2+?chelating dye, Fluo-4, in conjunction with a Fluorometric Imaging Plate Reader. The cells are plated out the day before the experiment onto poly-D-lysine-coated black 96 well plates. After the agonist addition, changes in intracellular Ca2+ concentrations are recorded, per second, for 3 min. Ligands are tested at 11 half-log concentrations from 10-10 to 10-4 M. BzATP or ATP concentrations corresponds to the EC70?values for each receptor to enable comparison of antagonist potencies across the multiple P2 receptor subtypes. A 438079 is added to the cell plate and ?uorescence data are collected for 3 min before the addition of agonist, subsequently, data are then collected for another 2 min. The pEC50 or pIC50 values are derived from a single curve ?t. |
分子量 | 418.49 |
分子式 | C19H18N2O5S2 |
CAS No. | 1014691-61-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 4.18 mg/mL (10 mM)
DMSO: 41.85 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.3895 mL | 11.9477 mL | 23.8954 mL | 59.7386 mL |
5 mM | 0.4779 mL | 2.3895 mL | 4.7791 mL | 11.9477 mL | |
10 mM | 0.239 mL | 1.1948 mL | 2.3895 mL | 5.9739 mL | |
DMSO | 20 mM | 0.1195 mL | 0.5974 mL | 1.1948 mL | 2.9869 mL |
50 mM | 0.0478 mL | 0.239 mL | 0.4779 mL | 1.1948 mL | |
100 mM | 0.0239 mL | 0.1195 mL | 0.239 mL | 0.5974 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
GSK0660 1014691-61-2 DNA Damage/DNA Repair Metabolism PPAR inhibit Inhibitor GSK 0660 Peroxisome proliferator-activated receptors GSK-0660 inhibitor