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Etonogestrel

Etonogestrel

产品编号 T2567   CAS 54048-10-1
别名: 3-Oxodesogestrel, 3-keto-Desogestrel, 依托孕烯, Nexplanon, Implanon

Etonogestrel (Implanon) 是孕激素去氧孕烯的生物活性代谢物,与靶器官中的孕酮受体和雌激素受体高亲和力地结合,可诱导FKBP51mRNA 和蛋白表达。它是一种甾体孕激素,用作激素避孕药。

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Etonogestrel Chemical Structure
Etonogestrel, CAS 54048-10-1
规格 价格/CNY 货期 数量
1 mg ¥ 196 现货
5 mg ¥ 453 现货
10 mg ¥ 638 现货
25 mg ¥ 1,290 现货
50 mg ¥ 2,570 现货
100 mg ¥ 3,950 现货
200 mg ¥ 5,630 现货
500 mg ¥ 8,670 现货
1 g ¥ 11,300 现货
1 mL * 10 mM (in DMSO) ¥ 628 现货
产品目录号及名称: Etonogestrel (T2567)
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纯度: 99.69%
纯度: 99.59%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Etonogestrel (Implanon) is a steroidal progestin used as hormonal contraceptives.
体外活性 Etonogestrel induces FKBP51 mRNA and protein expression in cultured HESCs[1].
激酶实验 First, 0.5 mL tissue homogenate is diluted with 1 mL water. Then, to this mixture, 2.5 mL ethanol and 1.5 mL chloroform (all reagents chilled) are added and shaken for 1 min at 4°C, then centrifuged. The enzyme activity in the supernatant is determined. The assay mixture contained 1.2 mL sodium pyrophosphate buffer (0.025 M, pH 8.3), 0.1 mL 186 mM phenazine methosulfate (PMS), 0.3 mL 30 mM Nitroblue tetrazolium (NBT), and 0.2 mL of nicotinamide adenine dinucleotide (NADH), and appropriately diluted enzyme preparation and water in a total volume of 3 mL. Reaction is initiated by the addition of NADH. After incubation at 30°C for 90 min, the reaction is stopped by the addition of 1 mL glacial acetic acid. The reaction mixture is stirred vigorously and shaken with 4 mL n-butanol. The intensity of the chromogen in the butanol layer is measured at 560 nm against a butanol blank. A system without enzyme served as control. One unit of enzyme activity is defined as 50% inhibition of NBT reduction in 1 min under the assay conditions.
细胞实验 Thawed HESCs are grown to confluence in basal medium (BM), a phenol red-free 1:1 v/v mix of Dulbecco's MEM/Ham's F-12, with 100U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml fungizone complex supplemented with 10% charcoal-stripped calf serum. Confluent HESCs are incubated in parallel in BM with 0.1% ethanol (vehicle control) or 10 nM E2(estradiol) or E2+100 nM P4(progesterone), or 100 nM of ETO(etonogestrel) or 100 nM of MPA(medroxyprogesterone acetate). After 7 days, the cultures are washed twice with 1X phosphate-buffered saline (PBS) to remove residual serum, then placed in a serum-free defined media (DM) comprising BM plus ITS+ (insulin, transferrin, selenious and linoleic acid) premix, 5 μM of FeSO4, 50 μM of ZnSO4, 1 nM of CuSO4, 20 nM of Na2SeO3 trace elements, 50 μg/ml of ascorbic acid and 50 ng/ml of recombinant epidermal growth factor for 24h. The cultures are washed twice with 1X PBS to remove the residual steroids and DM containing corresponding vehicle control or steroids is added to the cultures for 6h or 24h. At the end of incubation periods, HESCs are washed with ice-cold 1X PBS and stored at ?70°C until used for total RNA and protein extraction.(Only for Reference)
别名 3-Oxodesogestrel, 3-keto-Desogestrel, 依托孕烯, Nexplanon, Implanon
分子量 324.46
分子式 C22H28O2
CAS No. 54048-10-1

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 59 mg/mL(181.8 mM)

Ethanol: 64 mg/mL (197.25 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 3.082 mL 15.4102 mL 30.8204 mL 77.0511 mL
5 mM 0.6164 mL 3.082 mL 6.1641 mL 15.4102 mL
10 mM 0.3082 mL 1.541 mL 3.082 mL 7.7051 mL
20 mM 0.1541 mL 0.7705 mL 1.541 mL 3.8526 mL
50 mM 0.0616 mL 0.3082 mL 0.6164 mL 1.541 mL
100 mM 0.0308 mL 0.1541 mL 0.3082 mL 0.7705 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Tasdemir N, et al. Gynecol Endocrinol. 2012, 28(6):463-7. 2. Guzeloglu Kayisli O, et al. PLoS One. 2015, 10(10):e0137855.
Cridanimod Megestrol acetate U-46619 Ulipristal acetate Levonorgestrel Melengestrol acetate Drospirenone Ulipristal

相关化合物库

该产品包含在如下化合物库中:
抗癌药物库 抗癌上市药物库 核受体化合物库 FDA 上市药物库 内分泌激素分子库 药物功能重定位化合物库 人代谢物化合物库 抗卵巢癌化合物库 儿童药物库 FDA上市及药典收录分子库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Etonogestrel 54048-10-1 Endocrinology/Hormones Metabolism Others Estrogen Receptor/ERR Estrogen/progestogen Receptor Progesterone Receptor Endogenous Metabolite endometrial Metabolite stromal FK506-binding protein inhibit Estrogen NR3C3 Contraception Desogestrel 3-Oxodesogestrel 3-keto-Desogestrel progestin FKBP51 依托孕烯 Nexplanon Implanon FKBP Inhibitor HESC inhibitor

 

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