Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Etonogestrel (Implanon) 是孕激素去氧孕烯的生物活性代谢物,与靶器官中的孕酮受体和雌激素受体高亲和力地结合,可诱导FKBP51mRNA 和蛋白表达。它是一种甾体孕激素,用作激素避孕药。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 196 | 现货 | ||
5 mg | ¥ 453 | 现货 | ||
10 mg | ¥ 638 | 现货 | ||
25 mg | ¥ 1,290 | 现货 | ||
50 mg | ¥ 2,570 | 现货 | ||
100 mg | ¥ 3,950 | 现货 | ||
200 mg | ¥ 5,630 | 现货 | ||
500 mg | ¥ 8,670 | 现货 | ||
1 g | ¥ 11,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 628 | 现货 |
产品描述 | Etonogestrel (Implanon) is a steroidal progestin used as hormonal contraceptives. |
体外活性 | Etonogestrel induces FKBP51 mRNA and protein expression in cultured HESCs[1]. |
激酶实验 | First, 0.5 mL tissue homogenate is diluted with 1 mL water. Then, to this mixture, 2.5 mL ethanol and 1.5 mL chloroform (all reagents chilled) are added and shaken for 1 min at 4°C, then centrifuged. The enzyme activity in the supernatant is determined. The assay mixture contained 1.2 mL sodium pyrophosphate buffer (0.025 M, pH 8.3), 0.1 mL 186 mM phenazine methosulfate (PMS), 0.3 mL 30 mM Nitroblue tetrazolium (NBT), and 0.2 mL of nicotinamide adenine dinucleotide (NADH), and appropriately diluted enzyme preparation and water in a total volume of 3 mL. Reaction is initiated by the addition of NADH. After incubation at 30°C for 90 min, the reaction is stopped by the addition of 1 mL glacial acetic acid. The reaction mixture is stirred vigorously and shaken with 4 mL n-butanol. The intensity of the chromogen in the butanol layer is measured at 560 nm against a butanol blank. A system without enzyme served as control. One unit of enzyme activity is defined as 50% inhibition of NBT reduction in 1 min under the assay conditions. |
细胞实验 | Thawed HESCs are grown to confluence in basal medium (BM), a phenol red-free 1:1 v/v mix of Dulbecco's MEM/Ham's F-12, with 100U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml fungizone complex supplemented with 10% charcoal-stripped calf serum. Confluent HESCs are incubated in parallel in BM with 0.1% ethanol (vehicle control) or 10 nM E2(estradiol) or E2+100 nM P4(progesterone), or 100 nM of ETO(etonogestrel) or 100 nM of MPA(medroxyprogesterone acetate). After 7 days, the cultures are washed twice with 1X phosphate-buffered saline (PBS) to remove residual serum, then placed in a serum-free defined media (DM) comprising BM plus ITS+ (insulin, transferrin, selenious and linoleic acid) premix, 5 μM of FeSO4, 50 μM of ZnSO4, 1 nM of CuSO4, 20 nM of Na2SeO3 trace elements, 50 μg/ml of ascorbic acid and 50 ng/ml of recombinant epidermal growth factor for 24h. The cultures are washed twice with 1X PBS to remove the residual steroids and DM containing corresponding vehicle control or steroids is added to the cultures for 6h or 24h. At the end of incubation periods, HESCs are washed with ice-cold 1X PBS and stored at ?70°C until used for total RNA and protein extraction.(Only for Reference) |
别名 | 3-Oxodesogestrel, 3-keto-Desogestrel, 依托孕烯, Nexplanon, Implanon |
分子量 | 324.46 |
分子式 | C22H28O2 |
CAS No. | 54048-10-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 59 mg/mL(181.8 mM)
Ethanol: 64 mg/mL (197.25 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.082 mL | 15.4102 mL | 30.8204 mL | 77.0511 mL |
5 mM | 0.6164 mL | 3.082 mL | 6.1641 mL | 15.4102 mL | |
10 mM | 0.3082 mL | 1.541 mL | 3.082 mL | 7.7051 mL | |
20 mM | 0.1541 mL | 0.7705 mL | 1.541 mL | 3.8526 mL | |
50 mM | 0.0616 mL | 0.3082 mL | 0.6164 mL | 1.541 mL | |
100 mM | 0.0308 mL | 0.1541 mL | 0.3082 mL | 0.7705 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Etonogestrel 54048-10-1 Endocrinology/Hormones Metabolism Others Estrogen Receptor/ERR Estrogen/progestogen Receptor Progesterone Receptor Endogenous Metabolite endometrial Metabolite stromal FK506-binding protein inhibit Estrogen NR3C3 Contraception Desogestrel 3-Oxodesogestrel 3-keto-Desogestrel progestin FKBP51 依托孕烯 Nexplanon Implanon FKBP Inhibitor HESC inhibitor