Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Esonarimod (KE-298) 是一种具有抗风湿作用的化合物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 251 | 现货 | ||
2 mg | ¥ 367 | 现货 | ||
5 mg | ¥ 663 | 现货 | ||
10 mg | ¥ 990 | 现货 | ||
25 mg | ¥ 1,930 | 现货 | ||
50 mg | ¥ 2,890 | 现货 | ||
100 mg | ¥ 4,360 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 755 | 现货 |
产品描述 | Esonarimod (KE-298) is an antirheumatic drug. |
体外活性 | The IC50 of Esonarimod is 117.5 μg/mL. In RAW264.7 cells, Esonarimod (KE-298) (10 to 300 μg/mL) suppresses the production of NO in a dose-dependent manner. |
体内活性 | After repeated oral administration of Esonarimod (14C-KE-298), the radioactivity decreases rapidly and no tendency towards accumulation is found. |
激酶实验 | To test enzyme activity of NOS, the lysate from RAW264.7 cells (a protein concentration of 37.5 μg/200 μL) is incubated for 3 h at 37°C with 100 mM of L-arginine in the presence of Esonarimod (KE-298) and the conversion of L-arginine to nitrite is monitored. |
细胞实验 | For in vitro experiment, Esonarimod (KE-298) is dissolved in ethanol and diluted with culture medium or distilled water.RAW264.7 cells are used in this study. For NO production, RAW264.7 cells [2×105/0.2 mL of RPMI-1640 supplemented by 10% heat inactivated fetal bovine serum (FBS), penicillin G (100 U/mL), and streptomycin (100 μg/mL)] are stimulated with 100 ng/mL of Escherichia coli 026:B6 lipopolysaccharide in the presence of Esonarimod (KE-298) (0, 10, 30, 100, 200, 300 μg/mL) in 96 well plates and incubated 24 h at 37°C in an atmosphere of 5% CO2 in air. After incubation, the supernatants are collected and assayed for nitrite (NO2-) instead of NO[1]. |
动物实验 | Seven-week-old male Wistar rats is administered Esonarimod (5 mg/kg once daily) orally by gastric intubation. |
别名 | KE-298 |
分子量 | 280.34 |
分子式 | C14H16O4S |
CAS No. | 101973-77-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (214.03 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.5671 mL | 17.8355 mL | 35.671 mL | 89.1774 mL |
5 mM | 0.7134 mL | 3.5671 mL | 7.1342 mL | 17.8355 mL | |
10 mM | 0.3567 mL | 1.7835 mL | 3.5671 mL | 8.9177 mL | |
20 mM | 0.1784 mL | 0.8918 mL | 1.7835 mL | 4.4589 mL | |
50 mM | 0.0713 mL | 0.3567 mL | 0.7134 mL | 1.7835 mL | |
100 mM | 0.0357 mL | 0.1784 mL | 0.3567 mL | 0.8918 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Esonarimod 101973-77-7 Immunology/Inflammation IL Receptor KE298 KE-298 inhibit Inhibitor KE 298 inhibitor