Powder: -20°C for 3 years | In solvent: -80°C for 1 year
E-64 (Proteinase inhibitor E 64)是一种不可逆的半胱氨酸蛋白酶抑制剂,抑制papain 的IC50为 9 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 219 | 现货 | ||
5 mg | ¥ 395 | 现货 | ||
10 mg | ¥ 617 | 现货 | ||
25 mg | ¥ 1,090 | 现货 | ||
50 mg | ¥ 1,650 | 现货 | ||
100 mg | ¥ 2,460 | 现货 | ||
500 mg | ¥ 5,820 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 398 | 现货 |
产品描述 | E-64 (Proteinase inhibitor E 64) is an irreversible and specific cysteine protease inhibitor. The IC50 of E-64 for papain is 9 nM. |
靶点活性 | Cysteine protease:9 nM |
体外活性 | E-64 rapidly inhibits the activities of the cysteine proteinases cathepsins B, H and L and papain, while has no effect on the serine proteinases or the metalloproteinases. [2] E-64, as a specific inhibitor os cysteine proteases, effectively blocks in vitro ovarian cancer cell invasion. [3] In addition, E-64 also shows antiparasitic activity against Giardia lamblia excystation in vitro. [4] E-64 improves the preimplantation development of bovine somatic cell nuclear transfer embryos, which indicates another important implication of E-64. [5] |
体内活性 | E-64 induces a marked decrease in the number of spontaneous metastasis in M5076 tumor bearing mice, while has no effect on the formation of experimental metastasis. [6] E-64 also shows antiparasitic activity against Giardia lamblia excystation in infected mice. [4] |
激酶实验 | The Cathepsin Β activity is determined using Z-Arg-Arg-4mβNA as substrate with slight modifications. The crude extract is pre-incubated at 37°C for 5 min in 50 mM sodium acetate buffer, pH 5.0 containing 1 mM EDTA and 5 mM DTT. The substrate (final concentration, 100 μM) is added to make the final assay volume of 1 mL. The reaction mixture is incubated at 37°C for 30 min. The reaction is terminated by adding equal volume of stopping reagent containing Fast Garnet GBC salt (1 mg/mL), 10 mM pHMB and 50 mM EDTA, pH 6.0. The extraction of product, β-napthylamine (β-NA), is carried out with n-butanol. After complete layer separation, the absorbance is measured in n-butanol layer and activity is calculated using molar extinction coefficient of β-napthylamine solution as 31.5 M/cm per sec at 520 nm. One unit of enzyme activity is defined as the amount of enzyme liberating 1 μmol of βNA per minute at 37°C. The half maximal inhibitory concentration (IC50) is calculated by plotting the graph between the different concentration of E-64 and the % inhibition in cathepsin B activity. Here, IC50 indicates the concentration of the E-64 required to inhibit the parasitic cathepsin B activity by half[2]. |
别名 | Proteinase inhibitor E 64, E 64, E64 |
分子量 | 357.41 |
分子式 | C15H27N5O5 |
CAS No. | 66701-25-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 9 mg/mL (25.2 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 66 mg/mL (184.7 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 2.7979 mL | 13.9895 mL | 27.9791 mL | 69.9477 mL |
5 mM | 0.5596 mL | 2.7979 mL | 5.5958 mL | 13.9895 mL | |
10 mM | 0.2798 mL | 1.399 mL | 2.7979 mL | 6.9948 mL | |
20 mM | 0.1399 mL | 0.6995 mL | 1.399 mL | 3.4974 mL | |
DMSO | 50 mM | 0.056 mL | 0.2798 mL | 0.5596 mL | 1.399 mL |
100 mM | 0.028 mL | 0.1399 mL | 0.2798 mL | 0.6995 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
E-64 66701-25-5 Autophagy Microbiology/Virology Proteases/Proteasome Cysteine Protease Antibacterial Proteinase inhibitor E 64 Cathepsin E 64 Inhibitor Proteinase inhibitor E-64 inhibit Proteinase inhibitor E64 E64 Bacterial inhibitor