Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dacarbazine (DTIC-Dome) 是一种抗肿瘤剂,对黑色素瘤具有显着的活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 232 | 现货 | ||
100 mg | ¥ 384 | 现货 | ||
200 mg | ¥ 494 | 现货 | ||
500 mg | ¥ 695 | 现货 | ||
1 g | ¥ 840 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 198 | 现货 |
产品描述 | Dacarbazine (DTIC-Dome) is an antineoplastic agent. It has significant activity against melanomas. |
体外活性 | Treatment of Dacarbazine sensitizes melanomas to lysis of peptide-specific CTL and it is is mediated through Fas-independent pathway[2]. |
体内活性 | Treatment of combination of Axitinib and DTIC demonstrates significant antitumor activity against melanoma flank xenografts, reduces tumor cell proliferation and decreases the area of tumor necrosis and increases apoptosis. It also reduces meta-tasis-related factors and prolongs lifespan in mice[3]. |
细胞实验 | Apotosis assay: UACC903 (UACC) cells treated with or without DTIC (20 μM) for 48 h are incubated with agonist anti-Fas Ab, CH-11 (500 ng/ml), for 5 and 16 h, respectively. To distinguish whether death/apoptosis is mediated by FasR, blocking anti-Fas Ab ZB4 (2 mg/ml) is added in the corresponding groups. At the end of incubation, cells are harvested and stained with propidium iodide and FITC-annexin V (BD PharMingen) for detecting apoptosis according to the instruction provided by the company. Ten thousand cells from each group are collected, and dead and apoptotic cells are analyzed without gating using CellQuest software. (Only for Reference) |
别名 | Imidazole Carboxamide, 达卡巴嗪, DTIC-Dome |
分子量 | 182.18 |
分子式 | C6H10N6O |
CAS No. | 4342-03-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 3 mg/mL (16.46 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.4891 mL | 27.4454 mL | 54.8908 mL | 137.2269 mL |
5 mM | 1.0978 mL | 5.4891 mL | 10.9782 mL | 27.4454 mL | |
10 mM | 0.5489 mL | 2.7445 mL | 5.4891 mL | 13.7227 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Dacarbazine 4342-03-4 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair Nucleoside Antimetabolite/Analog DNA/RNA Synthesis B cell inhibit antineoplastic Inhibitor Imidazole Carboxamide T cell 达卡巴嗪 DTIC-Dome metastatic malignant melanoma inhibitor