Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Carmustine (bis-chloroethylnitrosourea) 是一种细胞周期性非特异性烷基化抗肿瘤剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 279 | 现货 | ||
10 mg | ¥ 393 | 现货 | ||
25 mg | ¥ 659 | 现货 | ||
50 mg | ¥ 828 | 现货 | ||
100 mg | ¥ 1,470 | 现货 | ||
200 mg | ¥ 2,280 | 现货 | ||
500 mg | ¥ 3,830 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 293 | 现货 |
产品描述 | Carmustine (bis-chloroethylnitrosourea) is a cell-cycle phase nonspecific alkylating antineoplastic agent. |
靶点活性 | DNA Alkylator: |
体外活性 | Carmustine is an antitumor chemotherapeutic agent. Carmustine (8, 80, and 800 μM) decreases N-acetyltransferase (NAT) activities for 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) in rat glial tumor cytosol and intact cells. The carmustine decreases the formation of DNA-AF adduct when the DNA-AF adduct increases in rat glial tumor cell. |
体内活性 | Carmustine (BCNU; 25 mg/kg, i.p.) causes higher levels of the rhe ratio of liver weight to body weight and plasma conjugated bilirubin, and lower biliary flow, oxidised glutation levels (GSSG) and reduced glutation (GSH)/GSSG values than control rats. |
激酶实验 | The determination of Acetyl-CoAdependent N-acetylation of 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) are performed. Incubation mixtures in the assay system consists of a total volume of 90 μL: glial tumor cells cytosols, diluted as required, in 50 μL of lysis buffer (20 mM Tris/HCl, pH 7.5, 1 mM DTT and 1 mM EDTA), 20 μL of an Acetyl-CoA recycling mixture of 50 mM Tris-HCl (pH7.5), 0.2 mM EDTA, 2 mM DTT, 15 mM acetylcamitine, 2U/mL carnitine acetyltransferase, and AF or PABA at specific concentrations. The reactions are started by addition of 20 μL of Acetyl-CoA. The control reactions have 20 μL distilled water in place of Acetyl-CoA. For the single point activity measurements, the final concentration of AF or PABA is 0.1 mM and AcCoA is 0.5 mM. The reaction mixtures with or without specific concentrations of Carmustine and lomustine are incubated at 37°C for 10 min and stopped with 50 μL of 20% trichloroacetic acid for the PABA reactions, and 100 μL of acetonitrile for the AF reactions. All of the reactions (experiments and controls) are run in triplicate |
动物实验 | Carmustine is formulated in corn oil.RatsIndividual rats are weighted prior to enter the study; their weights are recorded, and they are randomLy assigned to four groups. Group I (saline group); This group consists of 12 rats. These rats are injected with 2 mL/kg of saline intraperitoneally (IP) 48 h before the study, being included by the study 48 h later. Group II (corn oil group) consists of 15 rats. These rats are injected with 2 mL/kg of corn oil (vehicle) IP 48 h before the study. Group III (Carmustine group) consists of 16 rats. These rats are injected with 1 mL per day of saline IP, administered at the same hour of the day as a single-dose for 3 days. Twelve hours after the first dose of saline, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine. Group IV (trimetazidine group) consists of 12 rats. These rats are injected with 2.5 mg/kg per day of trimetazidine (TMZ) IP, administered at the same hour of the day as a single-dose for 3 days. 12 h after the first dose of TMZ, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine |
别名 | bis-chloroethylnitrosourea, Nitrumon, 卡莫司汀, Gliadel, BCNU, BiCNU, Carmubris |
分子量 | 214.05 |
分子式 | C5H9Cl2N3O2 |
CAS No. | 154-93-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (256.95 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.6718 mL | 23.359 mL | 46.7181 mL | 116.7951 mL |
5 mM | 0.9344 mL | 4.6718 mL | 9.3436 mL | 23.359 mL | |
10 mM | 0.4672 mL | 2.3359 mL | 4.6718 mL | 11.6795 mL | |
20 mM | 0.2336 mL | 1.168 mL | 2.3359 mL | 5.8398 mL | |
50 mM | 0.0934 mL | 0.4672 mL | 0.9344 mL | 2.3359 mL | |
100 mM | 0.0467 mL | 0.2336 mL | 0.4672 mL | 1.168 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Carmustine 154-93-8 DNA Damage/DNA Repair DNA Alkylator/Crosslinker DNA Alkylation bis-chloroethylnitrosourea Nitrumon 卡莫司汀 Inhibitor Gliadel BCNU BiCNU inhibit Carmubris inhibitor