Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CCT007093 是蛋白磷酸酶 1D(PPM1D Wip1)的抑制剂,IC50值为8.4 μM。Wip1 抑制可激活 mTORC1 通路,增强肝切除手术后肝细胞的增殖。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 153 | 现货 | ||
5 mg | ¥ 328 | 现货 | ||
10 mg | ¥ 545 | 现货 | ||
25 mg | ¥ 1,060 | 现货 | ||
50 mg | ¥ 1,920 | 现货 | ||
100 mg | ¥ 3,160 | 现货 | ||
500 mg | ¥ 6,870 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 342 | 现货 |
产品描述 | CCT007093 is an effective PPM1D (WIP1) inhibitor (IC50: 8.4 μM). |
靶点活性 | PPM1D:8.4 μM |
体内活性 | CCT007093与紫杉醇联用,可协同抑制4种耐紫杉醇的三重阴性乳腺癌细胞系。CCT007093对过表达PPM1D的人肿瘤细胞系(MCF-7,KPL-1,和MCF-3B)的显著抑制具有选择性。通过激活p38激酶的活性,CCT007093可诱导细胞死亡。CCT007093对乳腺癌细胞和皮肤转化的异位表达Wip1的角质细胞的细胞凋亡具有选择性促进作用,同时减弱Wip1裸细胞和皮肤角质细胞模型中UV介导的凋亡应答。 |
激酶实验 | In vitro phosphatase assay: Recombinant PPM1D (20-50?pmol) is diluted in Tris buffer (50?mM, pH 8), NaCl (100?mM), β-mercaptoethanol (1?mM) or DTT (1?mM) and treated with MnCl2 (0, 1, 10 and 20?mM) or MgCl2 (0 and 40?mM). Where appropriate, inhibitors of PPM1D (10-50?μM) are added and the assay mix incubated for 30?min at room temperature. Recombinant phospho-P38 (200?pmol) is then added and the mixture incubated at 37°C for 1?h. The reaction is quenched by the addition of excess ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulphate-sample loading buffer and boiling for 5?min at 95°C followed by gel electrophoresis and western blotting. |
细胞实验 | Cells are transfected with a pSUPER plasmid and an additional plasmid expressing the blasticidin resistance gene (pEFBsd) in a molar ratio of 10:1. Cells are plated in 10?cm plates 24?h after transfection. Blasticidin selection (5?μg/ml) is initiated 48?h post-transfection and replenished every 3 days. Colonies are fixed in methanol and stained with crystal violet after 14 days. Colonies are quantified on a Colcount and the surviving fraction (SF) determined.(Only for Reference) |
分子量 | 272.39 |
分子式 | C15H12OS2 |
CAS No. | 176957-55-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
CCT007093 176957-55-4 Apoptosis Autophagy Metabolism Phosphatase inhibit CCT 007093 CCT-007093 Inhibitor inhibitor