首页 工具
登录
购物车
Apoptozole

Apoptozole

产品编号 T3293   CAS 1054543-47-3
别名: Apoptosis Activator VII

Apoptozole (Apoptosis Activator VII) 是一种Hsc70和Hsp70的 ATPase 结构域抑制剂,可诱导凋亡,Kd 值分别为 0.21 和 0.14 μM。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
Apoptozole Chemical Structure
Apoptozole, CAS 1054543-47-3
规格 价格/CNY 货期 数量
2 mg ¥ 279 现货
5 mg ¥ 439 现货
10 mg ¥ 628 现货
25 mg ¥ 1,330 现货
50 mg ¥ 2,620 现货
100 mg ¥ 3,830 现货
200 mg ¥ 5,480 现货
1 mL * 10 mM (in DMSO) ¥ 672 现货
产品目录号及名称: Apoptozole (T3293)
点击图片重新获取验证码
选择批次  
纯度: 98.02%
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Apoptozole (Apoptosis Activator VII) is an inhibitor of the ATPase domain of Hsc70 and Hsp70, and can induce apoptosis.
靶点活性 HSP70:0.14 μM(Kd), HSC70:0.21 μM(Kd)
体外活性 Apoptozole induces caspase dependent apoptosis by blocking interaction of HSP70 with APAF-1, without affecting interactions of HSP70 with ASK1, JNK, BAX, and AIF[2]. However, apoptozole may form aggregates under aqueous conditions that could interact with HSP70 proteins in a non-specific manner, potentially leading to false positives and inconsistent data[3].
体内活性 Apoptozole greatly retards tumor growth in mice xenografted with cancer cells without affecting mouse viability. The elimination half-life time (T1/2) of Az in blood is found to be significantly longer than that of Dox (8.04 versus 1.60 hr) and the time needed to reach a maximum concentration (Tmax) of Az is shorter than that of Dox (1.00 versus 4.00 hr)[2].
激酶实验 Inhibition of HER2/erbB2 tyrosine kinase activity: BT-474 cells are seeded on 24-well plates and cultured overnight. Mubritinib is then added at various concentrations. After incubation for 2 hours, the cells are harvested directly into sodium dodecyl sulfate (SDS)-sample buffer (200 μL). Aliquots containing equal amounts of total cell extract are run on 7.5% to 15% gradient SDS–polyacrylamide gel electrophoresis (PAGE). Following electrophoresis, proteins are transferred onto a polyvinylidene fluoride (PVDF) membrane, for western blot analysis using a relevant primary antibody. Detection of protein is accomplished by an enhanced chemiluminescent (ECL) detection method. The extent of tyrosine phosphorylation of HER2/erbB2 is measured by the LAS-1000 plus lumino-image analyser. The concentration of Mubritinib that inhibits HER2/erbB2 phosphorylation by 50% (IC50) is calculated from a dose–response curve generated by least-squares linear regression of the response using SAS software.
细胞实验 Several cancer cell lines (A549, lung adenocarcinoma epithelial cells; HeLa, cervical cancer cells; MDA-MB-231, breast cancer cells; HepG2, liver cancer cells) are treated with various concentrations (0-15 μM) of Az or compound 7 as a negative control for 18 hr. Cell viabilities are then determined using an MTT assay.(Only for Reference)
别名 Apoptosis Activator VII
分子量 625.56
分子式 C33H25F6N3O3
CAS No. 1054543-47-3

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 24 mg/mL (38.4 mM)

DMSO: 93 mg/mL (148.7 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 1.5986 mL 7.9928 mL 15.9857 mL 39.9642 mL
5 mM 0.3197 mL 1.5986 mL 3.1971 mL 7.9928 mL
10 mM 0.1599 mL 0.7993 mL 1.5986 mL 3.9964 mL
20 mM 0.0799 mL 0.3996 mL 0.7993 mL 1.9982 mL
DMSO 50 mM 0.032 mL 0.1599 mL 0.3197 mL 0.7993 mL
100 mM 0.016 mL 0.0799 mL 0.1599 mL 0.3996 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Williams DR, et al. Angew Chem Int Ed Engl. 2008, 47(39):7466-9. 2. Ko SK, et al. Chem Biol. 2015, 22(3):391-403. 3. Evans LE, et al. PLoS One. 2015, 10(10):e0140006.
Notopterol Embelin Brevilin A SC66 Tuvusertib Tubulin polymerization-IN-43 physalin F Mivebresib

相关化合物库

该产品包含在如下化合物库中:
内质网应激化合物库 抗代谢疾病化合物库 抗乳腺癌化合物库 铜死亡化合物库 抗COVID-19化合物库 细胞凋亡化合物库 细胞骨架化合物库 含氟化合物库 抑制剂库 代谢化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Apoptozole 1054543-47-3 Apoptosis Cytoskeletal Signaling Metabolism HSP domain Heat shock proteins inhibit Inhibitor Apoptosis Activator VII Hsp70 ATPase Hsc70 inhibitor

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼