keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Acarbose (BAY g 5421) 是一种具有口服具有活力 α-葡萄糖苷酶 (alpha-glucosidase) 抑制剂 (IC50=11 nM),是一种降糖药。它能提高磺脲类药物或胰岛素的降血糖作用。
产品描述 | Acarbose (BAY g 5421) is an inhibitor of α-Glucosidases with antihyperglycemic activity. |
体外活性 | Acarbose binds to and inhibits α-glucosidase, an enteric enzyme found in the brush border of the small intestines that hydrolyzes oligosaccharides and disaccharides into glucose and other monosaccharides. In addition, acarbose inhibits pancreatic α-amylase which hydrolyzes complex starches to oligosaccharides in the small intestines. |
细胞实验 | Cell viability is determined using the MTT assay. Cells are seeded in 24-well culture plates at a density of 2×104 cells/well, incubated for 48 h, treated with acarbose at varying concentrations (0.5, 1.0, 2.0, 3.0, and 5.0 μM) for 24 h; or pre-treated with TNF-α (20 ng/mL) for either 24 h or 48 h to evaluate the dose-dependent effects of acarbose on VSMC growth and viability, cultured with 0.5 mg/mL MTT at 37°C in a humidified atmosphere of 5% CO2 for another 4 h, and solubilized with isopropanol. The viable cell number varies directly with the concentration of formazan product measured spectrophotometrically at 563 nm. |
别名 | 阿卡波糖, BAY g 5421 |
分子量 | 645.6 |
分子式 | C25H43NO18 |
CAS No. | 56180-94-0 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 64.5 mg/mL (100 mM)
H2O: 64.5 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 1.5489 mL | 7.7447 mL | 15.4895 mL | 38.7237 mL |
5 mM | 0.3098 mL | 1.5489 mL | 3.0979 mL | 7.7447 mL | |
10 mM | 0.1549 mL | 0.7745 mL | 1.5489 mL | 3.8724 mL | |
20 mM | 0.0774 mL | 0.3872 mL | 0.7745 mL | 1.9362 mL | |
50 mM | 0.031 mL | 0.1549 mL | 0.3098 mL | 0.7745 mL | |
100 mM | 0.0155 mL | 0.0774 mL | 0.1549 mL | 0.3872 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Acarbose 56180-94-0 Metabolism Glucosidase mellitus oral antihyperglycemic insulin anti-diabetes BAY g5421 Inhibitor BAY g-5421 阿卡波糖 BAY g 5421 inhibit inhibitor